金黄色葡萄球菌对奶牛乳腺成纤维细胞TGF-β1/Smad信号通路及其转分化的影响

doi:10.11843/j.issn.0366-6964.2016.07.025

Abstract

Abstract:

This study aimed to investigate the effect of Staphylococcus aureus (S.aureus) on TGF-β1/Smad signaling pathway and transdifferentiation in bovine mammary fibroblasts (BMFB).BMFB were stimulated with heat-killed S.aureus (HKSA) at 10⁴，10⁵，10⁶，10⁷，10⁸ CFU•mL^-1.The transcription of TGF-β1，α-SMA and collagen-Ⅰ mRNA were detected by Real-time PCR after 24 h.The expression of α-SMA，collagen-Ⅰ and p-Smad2/3 protein were detected by Western blot.BMFB were pretreated with TGF-β1 receptor-specific inhibitor SB-431542，BMFB were stimulated with heat-killed S.aureus (HKSA) at 105 CFU•mL^-1.The transcription of α-SMA and collagen-Ⅰ mRNA were detected by Real-time PCR after 24 h.The expression of α-SMA，collagen-Ⅰ and p-Smad2/3 protein were detected by Western blot.Immunofluorescence was used to detect collagen-Ⅰ expression.The results showed that the transcription level of TGF-β1 mRNA，expression of α-SMA，collagen-Ⅰ and p-Smad2/3 increased significantly at different concentration after 24 h stimulation compared with unstimulated BMFB (P<0.05).The expression of TGF-β1，α-SMA，collagen-Ⅰ and p-Smad2/3 reached the maximal level at 105 CFU•mL^-1.The expression of α-SMA，collagen-Ⅰ and p-Smad2/3 were significantly reduced by pretreating BMFB with SB-431542 (P<0.01).The results indicate that TGF-β1/Smad signaling pathway plays an important role in S.aureus induced BMFB transdifferentiation.

CLC Number:

S852.3

YANG Bin，XU Dan-dan，SUN Zhi-peng，ZHAO Jia-qi，YAN Bo-wei，LI Xiao-ting，WU Rui. The Effect of Staphylococcus aureus on TGF-β1/Smad Signaling Pathway and Transdifferentiation in Bovine Mammary Fibroblasts[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(7): 1495-1501.

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The Effect of Staphylococcus aureus on TGF-β1/Smad Signaling Pathway and Transdifferentiation in Bovine Mammary Fibroblasts

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